stem cell factor Search Results


sox2  (Novus Biologicals)
90
Novus Biologicals sox2
A-C. Breast cancer cell lines were exposed to 20% or 1% O 2 for 24 h and NANOG (A), KLF4 (B), and <t>SOX2</t> (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, and normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . same cell line at 20% O 2 by Student's t test. D and E. HCC-1954 (D) and MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1α (sh1α) or HIF-2α (sh2α), or vectors encoding shRNAs targeting both HIF-1α and HIF-2α (DKD), were exposed to 20% or 1% O 2 for 24 h and RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . NTC at 20% O 2 ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . NTC at 1% O 2 by ANOVA. F . ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O 2 for 24 h and SOX2 mRNA was measured (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01 vs . NTC at 20% O2; ### P < 0.001 vs . NTC at 1% O2 by ANOVA. G and H . NTC and DKD subclones of HCC-1954 (G) and MCF-7 (H) were exposed to 20% or 1% O 2 for 48 h, whole cell lysates were prepared, and immunoblot assays were performed to analyze HIF-1α, HIF-2α, NANOG and KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O 2 for 48 h, and HIF-1α, NANOG and SOX2 immunoblot assays were performed.
Sox2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
sox2 - by Bioz Stars, 2026-04
90/100 stars
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stem cells  (Thermo Fisher)
98
Thermo Fisher stem cells
A-C. Breast cancer cell lines were exposed to 20% or 1% O 2 for 24 h and NANOG (A), KLF4 (B), and <t>SOX2</t> (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, and normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . same cell line at 20% O 2 by Student's t test. D and E. HCC-1954 (D) and MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1α (sh1α) or HIF-2α (sh2α), or vectors encoding shRNAs targeting both HIF-1α and HIF-2α (DKD), were exposed to 20% or 1% O 2 for 24 h and RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . NTC at 20% O 2 ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . NTC at 1% O 2 by ANOVA. F . ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O 2 for 24 h and SOX2 mRNA was measured (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01 vs . NTC at 20% O2; ### P < 0.001 vs . NTC at 1% O2 by ANOVA. G and H . NTC and DKD subclones of HCC-1954 (G) and MCF-7 (H) were exposed to 20% or 1% O 2 for 48 h, whole cell lysates were prepared, and immunoblot assays were performed to analyze HIF-1α, HIF-2α, NANOG and KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O 2 for 48 h, and HIF-1α, NANOG and SOX2 immunoblot assays were performed.
Stem Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
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cultrex stem cell qualified reduced growth factor basement membrane extract  (Trevigen)
94
Trevigen cultrex stem cell qualified reduced growth factor basement membrane extract
A-C. Breast cancer cell lines were exposed to 20% or 1% O 2 for 24 h and NANOG (A), KLF4 (B), and <t>SOX2</t> (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, and normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . same cell line at 20% O 2 by Student's t test. D and E. HCC-1954 (D) and MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1α (sh1α) or HIF-2α (sh2α), or vectors encoding shRNAs targeting both HIF-1α and HIF-2α (DKD), were exposed to 20% or 1% O 2 for 24 h and RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . NTC at 20% O 2 ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . NTC at 1% O 2 by ANOVA. F . ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O 2 for 24 h and SOX2 mRNA was measured (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01 vs . NTC at 20% O2; ### P < 0.001 vs . NTC at 1% O2 by ANOVA. G and H . NTC and DKD subclones of HCC-1954 (G) and MCF-7 (H) were exposed to 20% or 1% O 2 for 48 h, whole cell lysates were prepared, and immunoblot assays were performed to analyze HIF-1α, HIF-2α, NANOG and KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O 2 for 48 h, and HIF-1α, NANOG and SOX2 immunoblot assays were performed.
Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract, supplied by Trevigen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
cultrex stem cell qualified reduced growth factor basement membrane extract - by Bioz Stars, 2026-04
94/100 stars
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anti scf antibody  (Proteintech)
93
Proteintech anti scf antibody
A-C. Breast cancer cell lines were exposed to 20% or 1% O 2 for 24 h and NANOG (A), KLF4 (B), and <t>SOX2</t> (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, and normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . same cell line at 20% O 2 by Student's t test. D and E. HCC-1954 (D) and MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1α (sh1α) or HIF-2α (sh2α), or vectors encoding shRNAs targeting both HIF-1α and HIF-2α (DKD), were exposed to 20% or 1% O 2 for 24 h and RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . NTC at 20% O 2 ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . NTC at 1% O 2 by ANOVA. F . ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O 2 for 24 h and SOX2 mRNA was measured (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01 vs . NTC at 20% O2; ### P < 0.001 vs . NTC at 1% O2 by ANOVA. G and H . NTC and DKD subclones of HCC-1954 (G) and MCF-7 (H) were exposed to 20% or 1% O 2 for 48 h, whole cell lysates were prepared, and immunoblot assays were performed to analyze HIF-1α, HIF-2α, NANOG and KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O 2 for 48 h, and HIF-1α, NANOG and SOX2 immunoblot assays were performed.
Anti Scf Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
anti scf antibody - by Bioz Stars, 2026-04
93/100 stars
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c kit  (Boster Bio)
91
Boster Bio c kit
A-C. Breast cancer cell lines were exposed to 20% or 1% O 2 for 24 h and NANOG (A), KLF4 (B), and <t>SOX2</t> (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, and normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . same cell line at 20% O 2 by Student's t test. D and E. HCC-1954 (D) and MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1α (sh1α) or HIF-2α (sh2α), or vectors encoding shRNAs targeting both HIF-1α and HIF-2α (DKD), were exposed to 20% or 1% O 2 for 24 h and RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . NTC at 20% O 2 ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . NTC at 1% O 2 by ANOVA. F . ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O 2 for 24 h and SOX2 mRNA was measured (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01 vs . NTC at 20% O2; ### P < 0.001 vs . NTC at 1% O2 by ANOVA. G and H . NTC and DKD subclones of HCC-1954 (G) and MCF-7 (H) were exposed to 20% or 1% O 2 for 48 h, whole cell lysates were prepared, and immunoblot assays were performed to analyze HIF-1α, HIF-2α, NANOG and KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O 2 for 48 h, and HIF-1α, NANOG and SOX2 immunoblot assays were performed.
C Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
c kit - by Bioz Stars, 2026-04
91/100 stars
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mouse stem cell signaling  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc mouse stem cell signaling
A-C. Breast cancer cell lines were exposed to 20% or 1% O 2 for 24 h and NANOG (A), KLF4 (B), and <t>SOX2</t> (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, and normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . same cell line at 20% O 2 by Student's t test. D and E. HCC-1954 (D) and MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1α (sh1α) or HIF-2α (sh2α), or vectors encoding shRNAs targeting both HIF-1α and HIF-2α (DKD), were exposed to 20% or 1% O 2 for 24 h and RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . NTC at 20% O 2 ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . NTC at 1% O 2 by ANOVA. F . ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O 2 for 24 h and SOX2 mRNA was measured (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01 vs . NTC at 20% O2; ### P < 0.001 vs . NTC at 1% O2 by ANOVA. G and H . NTC and DKD subclones of HCC-1954 (G) and MCF-7 (H) were exposed to 20% or 1% O 2 for 48 h, whole cell lysates were prepared, and immunoblot assays were performed to analyze HIF-1α, HIF-2α, NANOG and KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O 2 for 48 h, and HIF-1α, NANOG and SOX2 immunoblot assays were performed.
Mouse Stem Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
mouse stem cell signaling - by Bioz Stars, 2026-04
93/100 stars
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scf  (Proteintech)
93
Proteintech scf
A-C. Breast cancer cell lines were exposed to 20% or 1% O 2 for 24 h and NANOG (A), KLF4 (B), and <t>SOX2</t> (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, and normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . same cell line at 20% O 2 by Student's t test. D and E. HCC-1954 (D) and MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1α (sh1α) or HIF-2α (sh2α), or vectors encoding shRNAs targeting both HIF-1α and HIF-2α (DKD), were exposed to 20% or 1% O 2 for 24 h and RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . NTC at 20% O 2 ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . NTC at 1% O 2 by ANOVA. F . ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O 2 for 24 h and SOX2 mRNA was measured (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01 vs . NTC at 20% O2; ### P < 0.001 vs . NTC at 1% O2 by ANOVA. G and H . NTC and DKD subclones of HCC-1954 (G) and MCF-7 (H) were exposed to 20% or 1% O 2 for 48 h, whole cell lysates were prepared, and immunoblot assays were performed to analyze HIF-1α, HIF-2α, NANOG and KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O 2 for 48 h, and HIF-1α, NANOG and SOX2 immunoblot assays were performed.
Scf, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/scf/product/Proteintech
Average 93 stars, based on 1 article reviews
scf - by Bioz Stars, 2026-04
93/100 stars
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glycerol chem impex  (Chem Impex International)
95
Chem Impex International glycerol chem impex
A-C. Breast cancer cell lines were exposed to 20% or 1% O 2 for 24 h and NANOG (A), KLF4 (B), and <t>SOX2</t> (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, and normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . same cell line at 20% O 2 by Student's t test. D and E. HCC-1954 (D) and MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1α (sh1α) or HIF-2α (sh2α), or vectors encoding shRNAs targeting both HIF-1α and HIF-2α (DKD), were exposed to 20% or 1% O 2 for 24 h and RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . NTC at 20% O 2 ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . NTC at 1% O 2 by ANOVA. F . ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O 2 for 24 h and SOX2 mRNA was measured (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01 vs . NTC at 20% O2; ### P < 0.001 vs . NTC at 1% O2 by ANOVA. G and H . NTC and DKD subclones of HCC-1954 (G) and MCF-7 (H) were exposed to 20% or 1% O 2 for 48 h, whole cell lysates were prepared, and immunoblot assays were performed to analyze HIF-1α, HIF-2α, NANOG and KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O 2 for 48 h, and HIF-1α, NANOG and SOX2 immunoblot assays were performed.
Glycerol Chem Impex, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glycerol chem impex - by Bioz Stars, 2026-04
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stem cell factor scf  (Boster Bio)
90
Boster Bio stem cell factor scf
PKD2/3 enhance MCs migration through upregulation of <t>SCF,</t> <t>CCL5</t> and CCL11 in prostate cancer cellsmRNA and the protein level of the scf , ccl5 and ccl11 were analyzed by real-time qPCR and ELISA in DU145 ( a ) and PC-3 M ( b ) cells transfected with siRNA of PKD2, PKD3. Data representing the means ± S.D. of three independent experiments was analyzed by one-way ANOVA for significance versus si-CTL. ***p < 0.001, **p < 0.01, *p < 0.05 versus si-CTL. c Knockdown efficiency of PKD2 and PKD3 in prostate cancer cells was verified by Western blotting. d DU145 cells were transfected with siRNA of PKD2, PKD3, the Conditional medium (CM) was collected to measure migration of P815 cells in response to SCF, CCL5, and CCL11 treatment by transwell assay. e Quantification were analyzed from data in D. ***p < 0.001 versus si-CTL by One-ANOVA tests
Stem Cell Factor Scf, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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rabbit anti scf  (Boster Bio)
90
Boster Bio rabbit anti scf
PKD2/3 enhance MCs migration through upregulation of <t>SCF,</t> <t>CCL5</t> and CCL11 in prostate cancer cellsmRNA and the protein level of the scf , ccl5 and ccl11 were analyzed by real-time qPCR and ELISA in DU145 ( a ) and PC-3 M ( b ) cells transfected with siRNA of PKD2, PKD3. Data representing the means ± S.D. of three independent experiments was analyzed by one-way ANOVA for significance versus si-CTL. ***p < 0.001, **p < 0.01, *p < 0.05 versus si-CTL. c Knockdown efficiency of PKD2 and PKD3 in prostate cancer cells was verified by Western blotting. d DU145 cells were transfected with siRNA of PKD2, PKD3, the Conditional medium (CM) was collected to measure migration of P815 cells in response to SCF, CCL5, and CCL11 treatment by transwell assay. e Quantification were analyzed from data in D. ***p < 0.001 versus si-CTL by One-ANOVA tests
Rabbit Anti Scf, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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endogenous bone marrow stem cells  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology endogenous bone marrow stem cells
PKD2/3 enhance MCs migration through upregulation of <t>SCF,</t> <t>CCL5</t> and CCL11 in prostate cancer cellsmRNA and the protein level of the scf , ccl5 and ccl11 were analyzed by real-time qPCR and ELISA in DU145 ( a ) and PC-3 M ( b ) cells transfected with siRNA of PKD2, PKD3. Data representing the means ± S.D. of three independent experiments was analyzed by one-way ANOVA for significance versus si-CTL. ***p < 0.001, **p < 0.01, *p < 0.05 versus si-CTL. c Knockdown efficiency of PKD2 and PKD3 in prostate cancer cells was verified by Western blotting. d DU145 cells were transfected with siRNA of PKD2, PKD3, the Conditional medium (CM) was collected to measure migration of P815 cells in response to SCF, CCL5, and CCL11 treatment by transwell assay. e Quantification were analyzed from data in D. ***p < 0.001 versus si-CTL by One-ANOVA tests
Endogenous Bone Marrow Stem Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
endogenous bone marrow stem cells - by Bioz Stars, 2026-04
93/100 stars
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ig hif 1 alpha polyclonal antibody proteintech  (Proteintech)
93
Proteintech ig hif 1 alpha polyclonal antibody proteintech
PKD2/3 enhance MCs migration through upregulation of <t>SCF,</t> <t>CCL5</t> and CCL11 in prostate cancer cellsmRNA and the protein level of the scf , ccl5 and ccl11 were analyzed by real-time qPCR and ELISA in DU145 ( a ) and PC-3 M ( b ) cells transfected with siRNA of PKD2, PKD3. Data representing the means ± S.D. of three independent experiments was analyzed by one-way ANOVA for significance versus si-CTL. ***p < 0.001, **p < 0.01, *p < 0.05 versus si-CTL. c Knockdown efficiency of PKD2 and PKD3 in prostate cancer cells was verified by Western blotting. d DU145 cells were transfected with siRNA of PKD2, PKD3, the Conditional medium (CM) was collected to measure migration of P815 cells in response to SCF, CCL5, and CCL11 treatment by transwell assay. e Quantification were analyzed from data in D. ***p < 0.001 versus si-CTL by One-ANOVA tests
Ig Hif 1 Alpha Polyclonal Antibody Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ig hif 1 alpha polyclonal antibody proteintech - by Bioz Stars, 2026-04
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Image Search Results


A-C. Breast cancer cell lines were exposed to 20% or 1% O 2 for 24 h and NANOG (A), KLF4 (B), and SOX2 (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, and normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . same cell line at 20% O 2 by Student's t test. D and E. HCC-1954 (D) and MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1α (sh1α) or HIF-2α (sh2α), or vectors encoding shRNAs targeting both HIF-1α and HIF-2α (DKD), were exposed to 20% or 1% O 2 for 24 h and RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . NTC at 20% O 2 ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . NTC at 1% O 2 by ANOVA. F . ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O 2 for 24 h and SOX2 mRNA was measured (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01 vs . NTC at 20% O2; ### P < 0.001 vs . NTC at 1% O2 by ANOVA. G and H . NTC and DKD subclones of HCC-1954 (G) and MCF-7 (H) were exposed to 20% or 1% O 2 for 48 h, whole cell lysates were prepared, and immunoblot assays were performed to analyze HIF-1α, HIF-2α, NANOG and KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O 2 for 48 h, and HIF-1α, NANOG and SOX2 immunoblot assays were performed.

Journal: Oncotarget

Article Title: Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells

doi: 10.18632/oncotarget.11743

Figure Lengend Snippet: A-C. Breast cancer cell lines were exposed to 20% or 1% O 2 for 24 h and NANOG (A), KLF4 (B), and SOX2 (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, and normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . same cell line at 20% O 2 by Student's t test. D and E. HCC-1954 (D) and MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1α (sh1α) or HIF-2α (sh2α), or vectors encoding shRNAs targeting both HIF-1α and HIF-2α (DKD), were exposed to 20% or 1% O 2 for 24 h and RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O 2 (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 vs . NTC at 20% O 2 ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs . NTC at 1% O 2 by ANOVA. F . ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O 2 for 24 h and SOX2 mRNA was measured (mean ± SEM; n = 3). * P < 0.05, ** P < 0.01 vs . NTC at 20% O2; ### P < 0.001 vs . NTC at 1% O2 by ANOVA. G and H . NTC and DKD subclones of HCC-1954 (G) and MCF-7 (H) were exposed to 20% or 1% O 2 for 48 h, whole cell lysates were prepared, and immunoblot assays were performed to analyze HIF-1α, HIF-2α, NANOG and KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O 2 for 48 h, and HIF-1α, NANOG and SOX2 immunoblot assays were performed.

Article Snippet: Blots were probed with HIF-1α (#610959, BD Biosciences, San Jose, CA), HIF-2α (#NB100-122, Novus Biologicals, Littleton, CO), ALKBH5 (#NBP1-82188, Novus Biologicals), NANOG (#NB100-588, Novus Biologicals), KLF4 (#NBP1-83940, Novus Biologicals), SOX2 (NBP1-42823, Novus Biologicals), ZNF217 (#NBP1-78189, Novus Biologicals), and Actin (#sc-1616, Santa Cruz Biotechnology, Dallas, TX) antibodies.

Techniques: Quantitative RT-PCR, Stable Transfection, Transfection, Expressing, Plasmid Preparation, Control, shRNA, Western Blot

Induction of BCSCs, pluripotency factors and m 6 A-regulating proteins by hypoxia

Journal: Oncotarget

Article Title: Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells

doi: 10.18632/oncotarget.11743

Figure Lengend Snippet: Induction of BCSCs, pluripotency factors and m 6 A-regulating proteins by hypoxia

Article Snippet: Blots were probed with HIF-1α (#610959, BD Biosciences, San Jose, CA), HIF-2α (#NB100-122, Novus Biologicals, Littleton, CO), ALKBH5 (#NBP1-82188, Novus Biologicals), NANOG (#NB100-588, Novus Biologicals), KLF4 (#NBP1-83940, Novus Biologicals), SOX2 (NBP1-42823, Novus Biologicals), ZNF217 (#NBP1-78189, Novus Biologicals), and Actin (#sc-1616, Santa Cruz Biotechnology, Dallas, TX) antibodies.

Techniques:

PKD2/3 enhance MCs migration through upregulation of SCF, CCL5 and CCL11 in prostate cancer cellsmRNA and the protein level of the scf , ccl5 and ccl11 were analyzed by real-time qPCR and ELISA in DU145 ( a ) and PC-3 M ( b ) cells transfected with siRNA of PKD2, PKD3. Data representing the means ± S.D. of three independent experiments was analyzed by one-way ANOVA for significance versus si-CTL. ***p < 0.001, **p < 0.01, *p < 0.05 versus si-CTL. c Knockdown efficiency of PKD2 and PKD3 in prostate cancer cells was verified by Western blotting. d DU145 cells were transfected with siRNA of PKD2, PKD3, the Conditional medium (CM) was collected to measure migration of P815 cells in response to SCF, CCL5, and CCL11 treatment by transwell assay. e Quantification were analyzed from data in D. ***p < 0.001 versus si-CTL by One-ANOVA tests

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Protein kinase Ds promote tumor angiogenesis through mast cell recruitment and expression of angiogenic factors in prostate cancer microenvironment

doi: 10.1186/s13046-019-1118-y

Figure Lengend Snippet: PKD2/3 enhance MCs migration through upregulation of SCF, CCL5 and CCL11 in prostate cancer cellsmRNA and the protein level of the scf , ccl5 and ccl11 were analyzed by real-time qPCR and ELISA in DU145 ( a ) and PC-3 M ( b ) cells transfected with siRNA of PKD2, PKD3. Data representing the means ± S.D. of three independent experiments was analyzed by one-way ANOVA for significance versus si-CTL. ***p < 0.001, **p < 0.01, *p < 0.05 versus si-CTL. c Knockdown efficiency of PKD2 and PKD3 in prostate cancer cells was verified by Western blotting. d DU145 cells were transfected with siRNA of PKD2, PKD3, the Conditional medium (CM) was collected to measure migration of P815 cells in response to SCF, CCL5, and CCL11 treatment by transwell assay. e Quantification were analyzed from data in D. ***p < 0.001 versus si-CTL by One-ANOVA tests

Article Snippet: Quantitative measurement of cytokines, including stem cell factor (SCF), Chemokine ligand 5 (CCL5), C-C motif chemokine 11(CCL11) and vascular endothelial growth factor (VEGF) secreted into conditioned medium was determined using ELISAs, according to the manufacturer’s protocol (BOSTER).

Techniques: Migration, Enzyme-linked Immunosorbent Assay, Transfection, Knockdown, Western Blot, Transwell Assay

PKD2 and PKD3 promote SCF, CCL5 and CCL11 expression through Erk1/2 signaling pathways. a-b Interaction of PKD2 or PKD3 with Erk1/2 was performed by co-IP assay in PC-3 M or DU145 prostate cancer cells. c-d DU145 cells ( c ) or PC-3 M cells ( d ) were transfected as indicated and treated with 100 nM PMA, phosphorylation and protein expression were detected by western blotting. e Overexpression efficiency of PKD2 and PKD3 in prostate cancer cells was verified by Western blotting. f ELISA were applied to measure SCF in conditional medium from DU145 cell transfected with GFP, GFP-PKD2, and GFP-PKD3 in present with or without Erk inhibitor PD98059(PD) treatment. g Real-time PCR was performed to analyze ccl5 expression in DU145 transfected with GFP, GFP-PKD2 and GFP-PKD3 plasmids followed by treatment with or without Erk inhibitor PD98059

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Protein kinase Ds promote tumor angiogenesis through mast cell recruitment and expression of angiogenic factors in prostate cancer microenvironment

doi: 10.1186/s13046-019-1118-y

Figure Lengend Snippet: PKD2 and PKD3 promote SCF, CCL5 and CCL11 expression through Erk1/2 signaling pathways. a-b Interaction of PKD2 or PKD3 with Erk1/2 was performed by co-IP assay in PC-3 M or DU145 prostate cancer cells. c-d DU145 cells ( c ) or PC-3 M cells ( d ) were transfected as indicated and treated with 100 nM PMA, phosphorylation and protein expression were detected by western blotting. e Overexpression efficiency of PKD2 and PKD3 in prostate cancer cells was verified by Western blotting. f ELISA were applied to measure SCF in conditional medium from DU145 cell transfected with GFP, GFP-PKD2, and GFP-PKD3 in present with or without Erk inhibitor PD98059(PD) treatment. g Real-time PCR was performed to analyze ccl5 expression in DU145 transfected with GFP, GFP-PKD2 and GFP-PKD3 plasmids followed by treatment with or without Erk inhibitor PD98059

Article Snippet: Quantitative measurement of cytokines, including stem cell factor (SCF), Chemokine ligand 5 (CCL5), C-C motif chemokine 11(CCL11) and vascular endothelial growth factor (VEGF) secreted into conditioned medium was determined using ELISAs, according to the manufacturer’s protocol (BOSTER).

Techniques: Expressing, Protein-Protein interactions, Co-Immunoprecipitation Assay, Transfection, Phospho-proteomics, Western Blot, Over Expression, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

PKD2 and PKD3 are required for SCF, CCL5, and CCL11 transcription. a Analysis of the binding site of p65(highlight in red) and AP1(highlight in blue) in the promoters of scf, ccl5 and ccl11 using UCSC software online. b Western blotting was used to ensure the knockdown effect. ChIP analysis of the binding of c-Jun ( c ), c-Fos (d) and NF-κB ( e ) to the scf, ccl5 and ccl11 gene promoter in PC-3 M cells depleted with siRNA of PKD2, PKD3. Student’s t-test , *p < 0.05, **p < 0.01 and ***p < 0.001 ( n = 3). Error bars indicate mean ± S.D.

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Protein kinase Ds promote tumor angiogenesis through mast cell recruitment and expression of angiogenic factors in prostate cancer microenvironment

doi: 10.1186/s13046-019-1118-y

Figure Lengend Snippet: PKD2 and PKD3 are required for SCF, CCL5, and CCL11 transcription. a Analysis of the binding site of p65(highlight in red) and AP1(highlight in blue) in the promoters of scf, ccl5 and ccl11 using UCSC software online. b Western blotting was used to ensure the knockdown effect. ChIP analysis of the binding of c-Jun ( c ), c-Fos (d) and NF-κB ( e ) to the scf, ccl5 and ccl11 gene promoter in PC-3 M cells depleted with siRNA of PKD2, PKD3. Student’s t-test , *p < 0.05, **p < 0.01 and ***p < 0.001 ( n = 3). Error bars indicate mean ± S.D.

Article Snippet: Quantitative measurement of cytokines, including stem cell factor (SCF), Chemokine ligand 5 (CCL5), C-C motif chemokine 11(CCL11) and vascular endothelial growth factor (VEGF) secreted into conditioned medium was determined using ELISAs, according to the manufacturer’s protocol (BOSTER).

Techniques: Binding Assay, Software, Western Blot, Knockdown

CRT0066101 reduces MCs recruitment and tumor angiogenesis in vivo. a Experimental setting. b C57BL6 mice bearing RM1 tumors were administered a daily vehicle [control group; 5% ( w / v ) dextrose] or CRT0066101 at 20 μM and 40 μM for 2 weeks (4 mice per group), then excised tumor images. c Tumor volume were represented at indicated day. d Immunohistochemistry staining for phospho-PKD, microvessel density (stained with CD31), and mast cells (stained with c-Kit). Representative image were shown in 400X under the microscope (Left panel). Quantification of the indicated parameter was analyzed among groups after treatment with CRT0066101 (Right panel). e Schematic model of the mechanistic role of PKD2 and PKD3 in tumor angiogenesis by regulating SCF-, CCL5-, and CCL11-mediated mast cell recruitment

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Protein kinase Ds promote tumor angiogenesis through mast cell recruitment and expression of angiogenic factors in prostate cancer microenvironment

doi: 10.1186/s13046-019-1118-y

Figure Lengend Snippet: CRT0066101 reduces MCs recruitment and tumor angiogenesis in vivo. a Experimental setting. b C57BL6 mice bearing RM1 tumors were administered a daily vehicle [control group; 5% ( w / v ) dextrose] or CRT0066101 at 20 μM and 40 μM for 2 weeks (4 mice per group), then excised tumor images. c Tumor volume were represented at indicated day. d Immunohistochemistry staining for phospho-PKD, microvessel density (stained with CD31), and mast cells (stained with c-Kit). Representative image were shown in 400X under the microscope (Left panel). Quantification of the indicated parameter was analyzed among groups after treatment with CRT0066101 (Right panel). e Schematic model of the mechanistic role of PKD2 and PKD3 in tumor angiogenesis by regulating SCF-, CCL5-, and CCL11-mediated mast cell recruitment

Article Snippet: Quantitative measurement of cytokines, including stem cell factor (SCF), Chemokine ligand 5 (CCL5), C-C motif chemokine 11(CCL11) and vascular endothelial growth factor (VEGF) secreted into conditioned medium was determined using ELISAs, according to the manufacturer’s protocol (BOSTER).

Techniques: In Vivo, Control, Immunohistochemistry, Staining, Microscopy